Laser Hatching:

Laser hatching is a new ART method in which laser beam is irradiated in a controlled manner to the zona pellucida, creating a gap that helps to remove the embryo from the shell and facilitates implantation and it is used for frozen embryo. This procedure is performed during preimplantation period. The egg is surrounded by a layer of protein sugar molecules called zona pellucida. This layer protects the egg and the embryo, and after the fertilization stage, it is one of the factors that guide the embryo from the fallopian tube to the uterus, which prevents the embryonic cells from separating from each other. Normally, the embryo leaves the shell before penetrating the endometrium and can be replaced in the endometrium. This separation causes the embryo to stick to the wall and increase the chances of pregnancy. In cases where in vitro fertilization is performed, some factors prevent the thinning of the zona pellucida and its rupture due to enzymes. According to studies, very rarely, an embryo can be damaged from the assisted hatching process.